Immobilization of Murine Anti-BMP-2 Monoclonal Antibody on Various Biomaterials for Bone Tissue Engineering
Figure 3
(a) Histological analysis of rat calvarial bone defects implanted with anti-BMP-2 mAb immobilized on scaffolds showing presence of vital bone in implantation sites. No evidence of bone formation was observed in sites implanted with isotype-matched control Ab. Collagen exhibited the most compression, followed by alginate, while titanium had the best tissue volume maintenance. (b) Histomorphometric analysis of rat calvarial bone defects implanted with anti-BMP-2 mAb immobilized on 3 different scaffolds. Histomorphometric analysis was performed on Trichrome-stained sections and percentage of new bone formation was quantified. No significant difference was observed between the proportions of new bone formation for each biomaterial (). .
