Mass Spectrometry in Pharmacokinetic Studies of a Synthetic Compound for Spinal Cord Injury Treatment

Figure 4

The IG20 is recovered from CNS tissues after deproteinization and lipid extraction. The mass spectrum of tissue homogenates and glycolipid extracts from spinal cord ((a); (b), (c)) or brain ((d); (e), (f)) is shown. In IG20 doped homogenates of spinal cord (a) or brain (d) no signal was obtained. The extraction of total glycolipids allowed detection of synthetic glycoside in spinal (b) and brain extracts (e). When tissue suspension was deproteinized by proteinase K prior to glycolipids extraction, a higher signal for IG20 compound was obtained ((c), (f)). Arrows correspond to mass-charge peak for IG20.

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(b)

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(f)