Dental Stem Cell Migration on Pulp Ceiling Cavities Filled with MTA, Dentin Chips, or Bio-Oss

Figure 1

Experimental strategy. A tooth slice is prepared by removing the root of third molars and a canal is formed by using a handmade metal base. The root canal is filled with a reagent commonly used in endodontics such as MTA, Bio-Oss, or dentin chips; electron microscopy was performed to assess their surface morphology. The different materials were seeded with dental mesenchymal stem cells, cultured for 72 hours and studied by confocal microscopy; the state and the number of the cells were recorded and further analyzed.