Dental Stem Cell Migration on Pulp Ceiling Cavities Filled with MTA, Dentin Chips, or Bio-Oss

Figure 2

(a) The surface structural organization of each specimen was examined by scanning electron microscopy (magnification ×2200). All three reagents, MTA, Bio-Oss, and Dentin chips, offer rough surface which is adequate for cell adhesion with dentin chips showing the highest degree of coarseness followed by Bio-Oss. (b) Representative images of the cell cultures on the surface of the different dental substances by confocal microscopy. The cells were cultured for 72 hours on the provided specimens and fixed and stained with Phalloidin to depicture the cytoskeleton of the dental mesenchymal stem cells (red) and DAPI for the nucleus (blue). The morphology and the clustering of the cells vary according to the applied material.

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