Steroid Anti-Inflammatory Effects Did Not Improve Organ Quality in Brain-Dead Rats

Table 1

Primer sequences used for Real-Time PCR.

Gene	Primers	Amplicon size (bp)
TNF-α	5′-GGCTGCCTTGGTTCAGATGT-3′	79
	5′-CAGGTGGGAGCAACCTACAGTT-3′
IL-1β	5′-CAGCAATGGTCGGGACATAGTT-3′	75
	5′-GCATTAGGAATAGTGCAGCCATCT-3′
IL-6	5′-CCAACTTCCAATGCTCTCCTAATG-3′	89
	5′-TTCAAGTGCTTTCAAGAGTTGGAT-3′
C3	5′-CAGCCTGAATGAACGACTAGACA-3′	96
	5′-TCAAAATCATCCGACAGCTCTATC-3′
MCP-1	5′-CTTTGAATGTGAACTTGACCCATAA-3′	78
	5′-ACAGAAGTGCTTGAGGTGGTTGT-3′
HO-1	5′-CTCGCATGAACACTCTGGAGAT-3′	74
	5′-GCAGGAAGGCGGTCTTAGC-3′
HSP-70	5′-GGTTGCATGTTCTTTGCGTTTA-3′	80
	5′-GGTGGCAGTGCTGAGGTGTT-3′
BAX	5′-GCGTGGTTGCCCTCTTCTAC-3′	74
	5′-TGATCAGCTCGGGCACTTTAGT-3′
Bcl-2	5′-CTGGGATGCCTTTGTGGAA-3′	70
	5′-TCAGAGACAGCCAGGAGAAATCA-3′