Role of Baicalin in Anti-Influenza Virus A as a Potent Inducer of IFN-Gamma

Figure 2

Effects of BA on IFN-γ production in A/PR/8/34-infected mice. Mice were treated as described in Figure 1. PBMC, CD4⁺, and CD8⁺ T cells and NK cells were then sorted on the seventh day. (a) 7 days after infection, IFN-γ in mice serum was calculated by ELISA, and the concentrations were determined from a standard curve. (b) Spot numbers of IFN-γ positive cells were obtained with PBMC from mice survived on the seventh day. cells were used per well, and experiments were performed in triplicate for each condition. (c) Total RNA was isolated from CD4⁺ and CD8⁺ T cells and NK cells and subjected to RT-PCR. The RT-PCR products of mice IFN-γ were analyzed on 1% agarose gel electrophoresis. (d) The cytosol protein was obtained from mice sorted CD4⁺ and CD8⁺ T cells and NK cells and analyzed by Western Blotting. ^#Levels of significance of against untreated mice; against control A/PR/8/34-infected mice; against control infected mice.

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