Yeast Actin-Related Protein ARP6 Negatively Regulates Agrobacterium-Mediated Transformation of Yeast Cell

Figure 1

Knockout of ARP6 increases AMT efficiency. (a) Transformation efficiency comparison between AMT and LiAc transformation. (b) The transformation efficiencies of WT and arp6Δ under different conditions. The arp6Δ mutant consistently increased transformation efficiencies as compared to WT with different input numbers and ratios of yeast to Agrobacterium. (c) The plasmids containing ARP6 that were introduced into yeast before AMT. (d) Complementation and overexpression assays. All the results were averaged from three independent experiments. Error bars present for SD. by -test.

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