Identification of the Alternative Splicing of the UL49 Locus of Human Cytomegalovirus

Figure 2

Agarose gel electrophoresis of differential transcripts of alternatively spliced UL49 cDNAs. Lane M: DNA Marker; Lane 1: cells uninfected by virus; Lanes 2 to 7: RT-PCR from HCMV-infected HDFn poly(A) RNA at 2, 6, 12, 48, 72, and 96 h postinfection, respectively; Lane 8: poly(A) RNA harvested at 72 h postinfection in the presence of inhibitor of DNA replication phosphonoformic acid (100 μM); and Lane 9: poly(A) RNA harvested at 24 h postinfection in the presence of inhibitor of protein synthesis cyclohexamide (100 μg/mL) 1 h prior to infection. UL49X and UL49Y were indicated by arrows.