Nanoparticle Labeling of Bone Marrow-Derived Rat Mesenchymal Stem Cells: Their Use in Differentiation and Tracking

Figure 3

Differentiation of CPN-labeled MSCs into ((a), (b)) osteogenic lineage, ((c), (d)) adipogenic lineage, and ((e), (f)) chondrogenic lineage after culturing with induction medium. (a) Visualization of osteogenic lineage by Alizarin Red staining; (c) adipogenic lineage by Oil Red O staining; and (e) chondrogenic lineage by Alcian Blue staining under bright-field microscopy. ((b), (d), and (f)) Visualization of CPN labeling shown by using FITC filters since CPN had a similar emission and excitation wavelength to FITC. Red arrows denote some of the positive staining for the presence of CPNs after differentiation. Magnification bar: 20 μm.

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