Anti-EGFR Antibody Reduces Lung Nodules by Inhibition of EGFR-Pathway in a Model of Lymphangioleiomyomatosis

Figure 1

LAM/TSC cells invade mouse lymph nodes and lungs causing lung nodules. (a) LAM/TSC cells are detected by immunoreactivity to HLA-A, B, and C antibody (green labelling, arrows) in lymph nodes and PKH-26 fluorescence (red labelling) in nude mice 15 (), 30 (), and 60 () weeks after cell administration (DAPI: blue labelling). (b) Staining with hematoxylin and eosin shows nodules in mouse lungs 30 and 60 weeks after LAM/TSC cell administration. 15 weeks after cell administration, no nodules are observed. Scale bars: 200 μm. Higher magnification scale bars: 100 μm. (c) Percentage of mice with lung lesions 30 and 60 weeks after cell administration. Data are expressed as mean ± SEM. (d) Lung nodules from mice of 30 weeks after cell administration are stained with α-actin (green) and anti-human HLA-A, B, and C (orange) antibody. Colocalization of the markers appears yellow (arrows). Sections are stained with DAPI to show nuclei (blue). Scale bars: 20 μm. (e) EGFR is detected by immunoreactivity to EGFR antibody in lung nodules (green labelling) of nude mice 30 weeks after cell administration (DAPI: blue labelling). Scale bars: 20 μm.