CRISPR/CAS9-Mediated Genome Editing of miRNA-155 Inhibits Proinflammatory Cytokine Production by RAW264.7 Cells

Figure 2

Generation of miR-155 GKO clones. DNA sequencing results of wild type and different deletions of miR-155 genomic DNA were presented in (a). (b) RT-qPCR results of mature miR-155 in different clones. . (c) SHIP mRNA was quantitated by RT-qPCR from LPS-treated RAW264.7 cells or D4 or D6 clones. (d) RAW264.7 cells were stimulated by LPS (100 ng/mL) for 36 hours followed by western blotting for SHIP1. The numbers below SHIP1 indicate the normalized expression levels.

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