Role of B7-H4 siRNA in Proliferation, Migration, and Invasion of LOVO Colorectal Carcinoma Cell Line

Figure 6

Effect of B7-H4 siRNA on the protein expressions of CXCL12, CXCR4, p-JAK, JAK, p-STAT3, and STAT3 in LOVO cells. (a and d) Identification of CXCL12/CXCR4 and JAK/STAT signalling as regulatory targets of B7-H4. Gene set enrichment analysis (GSEA) identified significant association between B7-H4 and CXCL12/CXCR4 and JAK/STAT signaling pathway in both the TCGA colorectal cancer dataset and the multitumor dataset. (b and c) After 48 h of B7-H4 siRNA treatment, the protein expressions of CXCL12 and CXCR4 in cells were analyzed by western blot. GAPDH was also detected as the control of sample loading. (e and f) Western blot was performed to identify the protein levels of p-JAK2, JAK, p-STAT3, and STAT3, and GAPDH was also detected as the control of sample loading. compared with the control cells; compared with the mock cells; data are expressed as the mean ± SD, .