Hypoxia Induces a Metabolic Shift and Enhances the Stemness and Expansion of Cochlear Spiral Ganglion Stem/Progenitor Cells

Figure 2

SPC markers and BrdU incorporation were revealed by immunocytochemistry. (a) Secondary spheres derived from spiral ganglion SPCs express nestin, an intermediate filament protein predominantly expressed by neural stem cells. (b) Nanog expression was observed in the nuclei of the majority of sphere-forming cells. (c) BrdU incorporation was detected in the nuclei of spheres. The blue-fluorescent DAPI nucleic acid stain for visualization of nuclei. Original magnification ×200.

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