Hypoxia Induces a Metabolic Shift and Enhances the Stemness and Expansion of Cochlear Spiral Ganglion Stem/Progenitor Cells

Figure 4

(a) The effects of different oxygen tensions on the proliferation of cochlear spiral SPCs were examined using the WST-1 test. The proliferative capacities in the hypoxic groups significantly increased compared with the normoxic group at each time point. (b) Morphological changes of the spheres in each oxygen tension group were observed on 21 DIV. (c) Quantitative analysis of sphere sizes generated from single-cell cultures of SPCs undergoing different oxygen tension culturing was carried out on 21 DIV. Results are expressed as mean ± SEM with for each bar. Scale bar = 300 μm; indicates ; indicates .

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