Research Article

A Functional Variant at miR-520a Binding Site in PIK3CA Alters Susceptibility to Colorectal Cancer in a Chinese Han Population

Figure 1

Effect of the PIK3CA 3′-UTR polymorphism rs141178472 on PIK3CA expression. (a) The sequence complementarity of hsa-miR-520a and PIK3CA 3′-UTR is shown here. (b) Luciferase activity of plasmids containing rs141178472 T or C allele in 293T cells. PIK3CA 3′-UTR luciferase reporter plasmids were cotransfected with chemically synthesized mature hsa-miR-520a or negative control (NC) in 293T cells. (c) The PIK3CA mRNA levels in PBMCs of CRC patients were detected by qRT-PCR assay. , .
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