miR-27 and miR-125 Distinctly Regulate Muscle-Enriched Transcription Factors in Cardiac and Skeletal Myocytes

Table 1

List of the oligonucleotides sequences used in the qPCR assays. Note that all primers were designed using the Primer3 (http://biotools.umassmed.edu/bioapps/primer3_www.cgi) online tool, fixing the primer length to 100–200 nucleotides and an annealing temperature of 60°C. MgCl₂ concentration was always the same since SSOFast EvaGreen Master mix was used in all qPCR experiments.

Gapdh	Fw: 5′-TCTTGCTCAGTGTCCTTGCTGG-3′	180 pb
	Rv: 5′-TCCTGGTATGACAATGAATACGC-3′
-Actin	Fw: 5′-CCAGAGGCATACAGGGAC-3′	144 pb
	Rv: 5′-TGAGGAGCACCCTGTGCT-3′
Myocd	Fw: 5′-TTTTCAATTCCATCCCCAAC-3′	210 pb
	Rv: 5′-CCCAGGGATCTTTGGAATTT-3′
Mdfi	Fw: 5′-CAGGCTCTGAACAGCATTGA-3′	125 pb
	Rv: 5′-GGTTCTGAGAGGTGGTCGTG-3′
Mstn	Fw: 5′-GGCTCTTTGGAAGATGACGA-3′	188 pb
	Rv: 5′-GGAGTCTTGACGGGTCTGAG-3′
Runx1	Fw: 5′-TACCTGGGATCCATCACCTC-3′	164 pb
	Rv: 5′-GACGGCAGAGTAGGGAACTG-3′
Mef2c	Fw: 5′-GGGGTGAGTGCATAAGAGGAC-3′	288 pb
	Rv: 5′-AGAAGAAACACGGGGACTATGGG-3′
Mef2d	Fw: 5′-TCTCCCAGTCTACCCACTCG-3′	162 pb
	Rv: 5′-CAGGTGAACTGAAGGCTGGT-3′