Immunodiagnosis of Canine Visceral Leishmaniasis Using Mimotope Peptides Selected from Phage Displayed Combinatorial Libraries

Table 1

Diagnostic performance of synthetic peptides, L. infantum chagasi antigen, and EIE-LVC Kit in sera of dogs.

Diagnostic test	FN	FP	Se %	Sp %	PPV %	NPV %	AUC	AC
5^*	0/38	0/69	100.00	100.00	100.00	100.00	1.0000	1.0000
6^*	0/38	2/69	100.00	97.10	95.00	100.00	0.9985	0.9813
11^*	0/38	0/69	100.00	100.00	100.00	100.00	1.0000	1.0000
Pep-Mix^*	0/38	2/69	100.00	97.10	95.00	100.00	0.9968	0.9813
Ag^*	0/38	16/69	100.00	76.81	70.37	100.00	0.9851	0.8505
EIE-LVC^#	2/38	22/69	94.74	68.12	62.07	95.92	NA	0.7757

Samples from healthy dogs and dogs with canine visceral leishmaniasis, T. cruzi, E. canis, or B. canis.
^*Cut-off obtained by ROC curve.
^#Cut-off obtained according to the manufacturer.
NA: not applicable; FN: false negative; FP: false positive; Se: sensitivity; Sp: specificity; AUC: area under curve; PPV: positive predictive value; NPV: negative predictive value; AC: accuracy.