DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature

Table 2

Fragments used to synthesize the Ubp4^′′a gene; Ubp4^′′a1–Ubp4^′′a15 are used in the assembly protocol and Ubp4^′′aFor and Ubp4^′′aRev for PCR.

Ubp4^′′a1	TTCGCGGTGGGCCTCGAGAATCTAGGAAATTCCTGCTATATGAACTGCATTATCC
Ubp4^′′a2	CAGCTCATGCGTCCCTAAAATACACTGGATAATGCAGTTCATATAGCAGGAATTTCCTAG
Ubp4^′′a3	AGTGTATTTTAGGGACGCATGAGCTGACGCAGATCTTCCTTGACGATTCGTATGCGAA
Ubp4^′′a4	CCCTAGTTTAGAATTGATATTGATGTGCTTCGCATACGAATCGTCAAGGAAGA
Ubp4^′′a5	GCACATCAATATCAATTCTAAACTAGGGTCGAAAGGTATTTTAGCTAAATATTTCGCTCGTCTTGTAC
Ubp4^′′a6	TTGGAGCCATCTACCTGTTCCTTATACATCATATGTACAAGACGAGCGAAATATTTAGCT
Ubp4^′′a7	ATATGATGTATAAGGAACAGGTAGATGGCTCCAAAAAAATTTCGATTAGCCCAATCAAATTTAAGCT
Ubp4^′′a8	AACAGTGAGTTGACGGAACCACAGGCGAGCTTAAATTTGATTGGGCTAATCG
Ubp4^′′a9	CGCCTGTGGTTCCGTCAACTCACTGTTTAAAACAGCTTCACAGCAAGATTGTCAGGAATTTTGCC
Ubp4^′′a10	GATCTTCATGCAAACCGTCAAGCAAGAATTGGCAAAATTCCTGACAATCTTGCTGTGA
Ubp4^′′a11	AATTCTTGCTTGACGGTTTGCATGAAGATCTGAACCAGTGCGGTTCTAACCCC
Ubp4^′′a12	CCTCTTGGCTCAGTTCTTTCAACGGGGGGTTAGAACCGCACTGGTTCA
Ubp4^′′a13	CCGTTGAAAGAACTGAGCCAAGAGGCAGAAGCGAGGCGGGAAAAGCTGAGCCTC
Ubp4^′′a14	GTTCCCATTCAATACTACTGGCTATGCGGAGGCTCAGCTTTTCCCGCCTCGC
Ubp4^′′a15	CGCATAGCCAGTAGTATTGAATGGGAACGCTTTCTGACCACGGATTTTAGCGTCATTGTG
Ubp4^′′a16	AGCGAGAGGCATATTGCCCTTGAAAGAGGTCCACAATGACGCTAAAATCCGTGG
Ubp4^′′a17	GACCTCTTTCAAGGGCAATATGCCTCTCGCTTAAAATGCAAAGTATGCAGCCACACTTCA
Ubp4^′′aFor	GGGGGAATTCGATATGTTCGCGGTGGGCCTCGAGAATC
Ubp4^′′bRev	GGGGGGATCCTTATGAAGTGTGGCTGCATACTTTGCATTTTAAG