Synergistic Activity of Carfilzomib and Panobinostat in Multiple Myeloma Cells via Modulation of ROS Generation and ERK1/2

Figure 2

Combination of carfilzomib and LBH589 induced enhanced apoptosis in MM cells. RPMI 8226 cells were exposed to carfilzomib (40 nM) and/or LBH589 (4 nM) for 24 h (upper panel) or 48 h (low panel) followed by Annexin V-FITC/PI double staining and flow cytometry analysis. The percentage of apoptotic cells (24 h) in Cont., LBH589, CFZ, and LBH589 + CFZ group was 6.7% ± 0.9%, 18.8% ± 2.8%, 30.0% ± 3.5%, and 56.6% ± 8.8, respectively. The value (48 h) was 6.8% ± 1.5%, 35.9% ± 3.8%, 48.3% ± 3.8%, and 81.5% ± 5.0, respectively. versus control group; . The data shown are representative of three independent experiments. Cont., control. CFZ, carfilzomib.