Aquaporin 5 Expression in Mouse Mammary Gland Cells Is Not Driven by Promoter Methylation

Figure 1

Analysis of AQP5 expression in nondividing EpH4 cells treated with Matrigel. (a) Shown is the average concentration of trypsinized cells [cells/mL] counted with a Coulter Counter from 4 different experiments. Averages are represented as crosses with confidence intervals; ; alpha = 0.05. Treatments were performed such that the age of the cells from seeding to the time of extraction was the same within one experiment. (b) Levels of AQP5 mRNA or protein measured after shorter or longer Dex treatment times. Cells were treated with Dex for 2 h, 6 h, 12 h, 24 h, and 72 h. Numbers inside bars represent experimental replicates; mRNA was normalized to GAPDH; protein was normalized to TUBB and shown relative to untreated samples. Bars represent averages and confidence intervals; ; alpha = 0.05. Significance was tested between controls (−Dex) and different Dex treatments, with asterisks denoting .

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