Research Article
Optimization of Fermentation Medium for Extracellular Lipase Production from Aspergillus niger Using Response Surface Methodology
Table 2
Coded levels and real values (in parentheses) for the BBD and lipase activity achieved after fermentation by A. niger G783.
| | Run |
Corn starch (g/L) |
Soybean meal (g/L) |
Soybean oil (g/L) | Lipase activity
(U/mL) |
| | 1 | 0 (10) | −1 (30) | 1 (15) | 1698 | | 2 | 1 (12) | 0 (35) | −1 (5) | 1866 | | 3 | 0 (10) | 0 (35) | 0 (10) | 2017 | | 4 | 0 (10) | 0 (35) | 0 (10) | 2093 | | 5 | −1 (8) | 0 (35) | −1 (5) | 1575 | | 6 | 0 (10) | 0 (35) | 0 (10) | 2014 | | 7 | 0 (10) | −1 (30) | −1 (5) | 1600 | | 8 | 0 (10) | 0 (35) | 0 (10) | 2113 | | 9 | 0 (10) | 0 (35) | 0 (10) | 2122 | | 10 | 1 (12) | −1 (30) | 0 (10) | 1744 | | 11 | 0 (10) | 1 (40) | 1 (15) | 1970 | | 12 | −1 (8) | 0 (35) | 1 (15) | 1837 | | 13 | −1 (8) | −1 (30) | 0 (10) | 1353 | | 14 | 0 (10) | 1 (40) | −1 (5) | 1807 | | 15 | −1 (8) | 1 (40) | 0 (10) | 1778 | | 16 | 1 (12) | 1 (40) | 0 (10) | 1754 | | 17 | 1 (12) | 0 (35) | 1 (15) | 1955 |
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