CF750-A33scFv-Fc-Based Optical Imaging of Subcutaneous and Orthotopic Xenografts of GPA33-Positive Colorectal Cancer in Mice

Figure 6

CF750-A33scFv-Fc-based optical imaging-directed orthotopic tumor tissue dissection. LS174T tumor tissues derived from xenografts were inoculated into colons of mice. Once the tumor tissues were palpable, the mice () were injected with CF750-A33scFv-Fc (100 μg) followed by scanning and tumor tissue dissection at 5 h after injection. Before laparotomy, the mice were first imaged using an optical imaging system with white light (a) and NIR filter settings (b). Next, laparotomy was performed, and the excrescent tumor tissues in the colon were identified by an optical imaging system (c and d). Finally, the orthotopic tumor tissues were removed under the guide of optical imaging (e). LS174T xenografts are indicated with blue circle.