Analysis of Coinfections with A/H1N1 Strain Variants among Pigs in Poland by Multitemperature Single-Strand Conformational Polymorphism

Figure 1

MSSCP-based identification of mixed infections in five swine samples collected from three farms in Poland in 2011–2013. The following samples were amplified by PCR and their corresponding ssDNA was separated on a 10% polyacrylamide gel using MSSCP method under optimum electrophoretic conditions: a hemagglutinin gene fragment from the five isolates (sw1–sw5), a reference pandemic A/Mexico/4486/09 strain (P) and a seasonal A/Brisbane/59/2007 strain (S), and a sample from a patient diagnosed with a coinfection with both the pandemic and seasonal strains (M). The DNA was visualized by silver staining. The electrophoretic profile of the analyzed swine samples sw2–sw5 is similar to the coinfection profile.