Isolation, Culture, and Characterization of Chicken Cartilage Stem/Progenitor Cells

Figure 8

Osteogenic differentiation of CSPCs. (a)–(d) Following induction, the number of cells increased quickly and the nodules increased in number and became larger with prolonged induction. About 3 weeks later, nodules were observed after alizarin red staining. (e) RT-PCR assays revealed expression of osteoblast specific genes, including collagen type I, secreted phosphoprotein 1, at day 21 after induction.

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