The Macrophage Galactose-Type Lectin-1 (MGL1) Recognizes Taenia crassiceps Antigens, Triggers Intracellular Signaling, and Is Critical for Resistance to This Infection

Figure 8

MGL1^−/− mice recruit alternatively activated macrophages. Peritoneal macrophages were obtained from MGL1^−/− and MGL1^+/+ T. crassiceps-infected mice and processed for RT-PCR or flow cytometry analyses. (a) RT-PCR showing the expression of arginase-1, Ym1, RELM-α and iNOS on macrophages obtained at 8 weeks after infection with T. crassiceps. (b) Representative dot plot and histograms demonstrating increased expression of PD-L2, and IL-4Rα on MGL1^−/− macrophages. (c) Percentages of expression of different surface markers on peritoneal macrophages. (d) Dot plots from PECs showing the expression of MGL1 and MGL2 from uninfected (control) and 8 weeks-infected T. crassiceps MGL1^+/+ and MGL1^−/− mice, numbers on the quadrants indicate average percentage of positive cells. Values are the mean ± SE ( animals) and are representative of two independent experiments. comparing MGL1^−/− versus MGL1^+/+ mice at the same time point.

(a)

(b)

(c)

(d)