Runx2/miR-3960/miR-2861 Positive Feedback Loop Is Responsible for Osteogenic Transdifferentiation of Vascular Smooth Muscle Cells

Figure 3

Inhibition of miR-2861 or miR-3960 inhibited β-glycerophosphate-induced osteoblast differentiation of vascular smooth muscle cells. ALP activity and osteocalcin secretion (a) and Western blot analysis of Runx2 protein expression (b) in 10 mM β-glycerophosphate- (β-GP-) treated VSMCs transfected with anti-miR-C (control) or anti-miR-2861, anti-miR-C, or anti-miR-3960. Data are shown as mean ± s.e.m. .

(a)

(b)