Runx2/miR-3960/miR-2861 Positive Feedback Loop Is Responsible for Osteogenic Transdifferentiation of Vascular Smooth Muscle Cells

Figure 4

miR-2861 and miR-3960 posttranscriptionally repressed HDAC5 and Hoxa2 expression, respectively. ((a) and (b)) Quantitative RT-PCR analysis of mRNA levels of HDAC5 and Hoxa2 (left) and Western blot analysis of protein levels of HDAC5 and Hoxa2 (right) in VSMCs transfected with miR-C (control) or pre-miR-3960 (a), miR-C, or miR-3960 (b). For quantitative RT-PCR analysis, mRNA levels normalized to GAPDH. Data are shown as means ± s.e.m. For Western blot analysis, β-actin acts as a loading control.

(a)

(b)