Optimization of Culture Conditions for Production of the Anti-Leukemic Glutaminase Free L-Asparaginase by Newly Isolated Streptomyces olivaceus NEAE-119 Using Response Surface Methodology

Table 1

Experimental independent variables at two levels used for the production of L-asparaginase by Streptomyces sp. strain NEAE-119 using Plackett-Burman design.

Code	Variables	Levels
		−1	+1
	Temperature (°C)	30	35
	pH	7	9
	Incubation time (days)	5	7
	Inoculum size (%, v/v)	2	4
	Inoculum age (h)	24	36
	Agitation speed (rpm)	100	150
	Dextrose (g/L)	2	4
	Starch (g/L)	10	20
	L-asparagine (g/L)	5	10
	KNO3 (g/L)	1	3
	Yeast extract (g/L)	0	1
	K2HPO4 (g/L)	1	2
	MgSO4·7H2O (g/L)	0.1	0.5
	NaCl (g/L)	0.1	0.5
	FeSO4·7H2O (g/L)	0	0.01