BioMed Research International

Research Article

Optimization of Culture Conditions for Production of the Anti-Leukemic Glutaminase Free L-Asparaginase by Newly Isolated Streptomyces olivaceus NEAE-119 Using Response Surface Methodology

Table 2

Physiological and biochemical (phenotypic) characteristics of Streptomyces sp. strain NEAE-119. Data for reference species (Streptomyces olivaceus) were taken from Bergey’s Manual of Systematic Bacteriology: Volume 5: the Actinobacteria [40].
CharacteristicStreptomyces sp. strain NEAE-119Streptomyces olivaceus
Aerial mycelium on ISP medium 2GreyGrey
Substrate mycelium on ISP medium 2Yellowish grey; substrate pigment is not a pH indicatorGreyed yellow; substrate pigment is not a pH indicator
Production of diffusible pigmentNo diffusible pigment 
Spore chain morphologySpirals, with open spirals intergrading through flexuous spore chains suggestive of section RectiflexibilesSpirals, with open spirals intergrading through flexuous spore chains suggestive of section Rectiflexibiles.
Spore surfaceSmoothSmooth
Spore shapeSpherical or oval to ellipsoidal 
Melanin production on peptone-yeast extract iron agar (ISP 6 medium)−−
Melanin production on tyrosine agar (ISP 7 medium)−−
Melanin production on tryptone-yeast extract broth (ISP 1 medium)− 
Max NaCl tolerance (%, w/v)8 
Growth on sole carbon source (1%, w/v)
D(−) Fructose++
D(+) Xylose++
D(+) Galactose++
D(+) Glucose++
L-arabinose++
Ribose++
D(+) Mannose++
Sucrose++
Maltose++
Rhamnose++
Cellulose++
Trehalose++
Enzymes
Lecithinase activity+ 
-amylase (starch hydrolysis)+ 
Protease− 
Cellulase (growth on cellulose)+ 
Uricase+ 
Chitosanase+ 
Asparaginase+ 
Reduction of nitrates to nitrite+ 
Coagulation of milk+ 
Peptonization of milk+ 
Antimicrobial activities against
Candida albicans,  Bacillus subtilis,  Escherichia coli, 
 Pseudomonas aeruginosa,  
Klebsiella pneumonia,  
Rhizoctonia solani,  Fusarium oxysporum, 
 Aspergillus niger. 		− 
Staphylococcus aureus,   Alternaria solani,  
Bipolaris oryzae. 		+ 
+: positive; −: negative; blank cells: no data available. Growth temperature range (°C): 25–40, growth at pH 5–9.