BioMed Research International

Research Article

Optimization of Culture Conditions for Production of the Anti-Leukemic Glutaminase Free L-Asparaginase by Newly Isolated Streptomyces olivaceus NEAE-119 Using Response Surface Methodology

Table 3

Twenty-trial Plackett-Burman experimental design for evaluation of fifteen independent variables with coded values along with the observed L-asparaginase activity.
RunDummy1Dummy2Dummy3Dummy4L-asparaginase activity
(U/mL)
11−11−1−1−1−111−111−1−11111−19.793
2−111−111−1−11111−11−11−1−1−111.330
31−1−1−1−111−111−1−11111−11−112.867
41111−11−11−1−1−1−111−111−1−116.852
51−11−11−1−1−1−111−111−1−11115.181
6−1−1−1−111−111−1−11111−11−1120.610
711−111−1−11111−11−11−1−1−1−113.778
8−111−1−11111−11−11−1−1−1−11120.708
911−11−11−1−1−1−111−111−1−11116.552
10−1−1−111−111−1−11111−11−11−111.501
11−1−111−111−1−11111−11−11−1−16.946
1211−1−11111−11−11−1−1−1−111−140.479
13−1−1−1−1−1−1−1−1−1−1−1−1−1−1−1−1−1−1−119.016
14−11111−11−11−1−1−1−111−111−19.223
151−1−11111−11−11−1−1−1−111−1149.874
16−11−1−1−1−111−111−1−11111−112.790
17−1−11111−11−11−1−1−1−111−11121.293
181−111−1−11111−11−11−1−1−1−119.451
19111−11−11−1−1−1−111−111−1−1113.550
20−11−11−1−1−1−111−111−1−1111112.127
The “−1” sign corresponds to the minimum value and the “+1” sign corresponds to the maximum value of the input parameter range.