Optimization of Culture Conditions for Production of the Anti-Leukemic Glutaminase Free L-Asparaginase by Newly Isolated Streptomyces olivaceus NEAE-119 Using Response Surface Methodology
Table 5
Face-centered central composite design, representing the response of L-asparaginase production as influenced by temperature (), inoculum age (), and agitation speed () along with the predicted L-asparaginase production and residuals and the levels of variables.
(a)
Trials
Variables
L-asparaginase activity (U/mL)
Residuals
Experimental
Predicted
1
0
0
0
68.196
66.667
1.530
2
1
1
1
34.451
38.978
−4.528
3
1
0
0
55.406
54.083
1.323
4
0
0
−1
38.765
38.104
0.662
5
1
−1
−1
15.501
21.237
−5.737
6
0
0
0
67.658
66.667
0.992
7
1
1
−1
29.266
25.752
3.514
8
0
−1
0
53.242
56.407
−3.165
9
−1
1
1
65.753
58.786
6.967
10
0
0
0
68.313
66.667
1.646
11
−1
0
0
52.860
59.104
−6.244
12
0
0
0
70.076
66.667
3.410
13
−1
−1
1
39.671
41.955
−2.284
14
0
0
0
68.196
66.667
1.530
15
0
0
0
67.403
66.667
0.736
16
−1
1
−1
29.771
33.968
−4.197
17
0
0
1
54.375
59.958
−5.583
18
−1
−1
−1
17.230
11.472
5.758
19
0
1
0
65.323
67.080
−1.756
20
1
−1
1
45.556
40.129
5.427
(b)
Level
Temperature (°C)
Inoculum age (h)
Agitation speed (rpm)
−1
30
24
100
0
35
48
150
1
37
72
200
The measured L-asparaginase activity obtained under the optimal conditions obtained from FCCD was 68.59 U/mL.
