Optimization of Culture Conditions for Production of the Anti-Leukemic Glutaminase Free L-Asparaginase by Newly Isolated Streptomyces olivaceus NEAE-119 Using Response Surface Methodology

Table 6

Statistical analysis of face-centered central composite design showing coefficient values, main effect, -test, values and analysis of variance.

(a)

Variables	Coefficients	Main effect	-Stat	value
Intercept	66.667	133.33	35.0717	0.0000
	−2.510	−5.02	−1.4358	0.1816
	5.337	10.67	3.0520	0.0122
	10.927	21.85	6.2494	0.0001
	−4.495	−8.99	−2.2995	0.0443
	−2.898	−5.80	−1.4824	0.1690
	−1.416	−2.83	−0.7245	0.4854
	−10.073	−20.15	−3.0210	0.0129
	−4.923	−9.85	−1.4765	0.1706
	−17.636	−35.27	−5.2891	0.0004

(b)

	Analysis of variance (ANOVA)
	df	SS	MS	-test	Significance ( value)
Regression	9	5854.7578	650.5286	21.2771
Residual	10	305.7400	30.5740
Total	19	6160.4978

: the coded value of temperature, : the coded value of inoculum age, and : the coded value of agitation speed.
: Student's test; : corresponding level of significance; df: degree of freedom; SS: sum of squares; MS: mean sum of squares; : Fishers's function; Significance : corresponding level of significance.
Multiple 0.9748, square 0.9503, and adjusted square 0.9057.