Evaluation of the Inflammatory Response in Macrophages Stimulated with Exosomes Secreted by Mycobacterium avium-Infected Macrophages

Figure 5

Analysis of caspase expression by western blotting. Caspases 3, 6, and 8 in macrophages treated with (−)exosomes (50 μg/mL), (+)exosomes (50 μg/mL), and M. avium (MOI of 10) were analyzed by western blotting. Controls included macrophages treated with LPS (50 ng/mL) or incubated with medium alone (mean ± SEM, 3 independent experiments). compared with the medium alone control.