Evaluation of the Inflammatory Response in Macrophages Stimulated with Exosomes Secreted by Mycobacterium avium-Infected Macrophages

Figure 6

Apoptosis of macrophages treated with different stimuli as detected by flow cytometry. (a) Macrophages were treated with LPS (50 ng/mL), (−)exosomes (50 μg/mL), (+)exosomes (50 μg/mL), and M. avium (MOI of 10), respectively, for 24 h. (b) Quantification of apoptosis and necrosis, with asterisks indicating the values for which significant differences were observed. Results presented as mean ± SEM (); results are representative of three separate experiments, compared with medium alone.

(a)

(b)