Evaluation of the Inflammatory Response in Macrophages Stimulated with Exosomes Secreted by Mycobacterium avium-Infected Macrophages

Table 1

Increased expression of CD40, CD80, CD81, CD86, CD195, and HLA-DR by macrophages treated with (+)exosomes, compared with other stimuli.

Treatment^a	Surface molecule expression (median MFI, 25th and 75th percentile MFI)^b
	CD25	CD32	CD40	CD80	CD81	CD86	CD163	CD195	HLA-DR
Medium	19 (13, 29)	35 (29, 40)	99 (88, 109)	105 (98, 112)	109 (100, 121)	155 (144, 164)	98 (79, 111)	48 (41, 55)	100 (93, 113)
LPS	18 (11, 33)	38 (32, 44)	115^* (88, 128)	166 (156, 188)	154^* (139, 167)	223^* (209, 240)	97 (88, 104)	49 (40, 59)	106^* (100, 123)
(−)Exo	19 (13, 37)	40 (33, 50)	99 (75, 108)	105 (99, 123)	105 (95, 121)	150 (141, 162)	99 (90, 112)	67 (55, 77)	102 (90, 111)
(+)Exo	20 (17, 33)	54 (46, 89)	119^* (109, 131)	179^* (126, 211)	156^* (145, 173)	224^* (199, 245)	104 (85, 111)	91^* (83, 111)	132^* (109, 138)
M. avium	22 (18, 40)	82^* (70, 112)	122^* (115, 145)	201^* (190, 222)	179^* (157, 195)	279^* (258, 301)	111^* (88, 117)	68 (47, 70)	143^* (127, 155)

Medium, incubated in medium only; LPS, activated by LPS; (−)exo, treated with (−)exosomes; (+)exo, treated with (+)exosomes; M. avium, infected with M. avium.
^bValues are based on five independent experiments. MFIs of isotype controls were subtracted. Values in parentheses are the 25th and 75th percentile MFIs, respectively. compared with medium alone (Friedman test and Dunn’s multiple comparison test).