Research Article
Binding of Carbonic Anhydrase IX to 45S rDNA Genes Is Prevented by Exportin-1 in Hypoxic Cells
Figure 1
Analysis of the putative nuclear localization and nuclear export signals in CA IX. (a) EGFP protein or its fusions with a canonical NLS from SV40 Ta or with the region encompassing the putative NLS of the CA IX protein sequence (amino acid positions 434–459), as indicated, were expressed in HEK293 cells and visualized by confocal fluorescence microscopy. Arrowheads indicate cells, in which the EGFP protein fused to the putative CA IX NLS was almost exclusively nuclear. White bars: 10 μm. (b) In the upper panels, EGFP protein or its fusions with a canonical NES from PKIα or with the putative NES-containing region of the CA IX protein sequence (amino acid positions 412–429), as indicated, were expressed in HEK293 cells and visualized by confocal fluorescence microscopy. In the lower panels, transfected cells were treated with leptomycin B (LMB) to inhibit XPO1-mediated nuclear export. White bars: 10 μm.