Redesigning Protein Cavities as a Strategy for Increasing Affinity in Protein-Protein Interaction: Interferon-γ Receptor 1 as a Model

Figure 7

Melting temperatures of selected IFNγR1 variants determined by thermal-based shift assay. Plotted are normalized data of reference-subtracted fluorescence intensities of IFNγR1 WT, V35L, N96W, and . The melting temperatures () of IFNγR1 variants were determined from the first derivatives of the curves plotted in the figure: 55°C for WT, 53°C for V35L, 49°C for N96W, and 48°C for . The values determined by temperature-dependent CD spectra and thermal-based shift assay are within 1°C the same.