Research Article

Production and Characterization of Lipases by Two New Isolates of Aspergillus through Solid-State and Submerged Fermentation

Table 2

Full Factorial Design (32) used to determine the influence of pH and temperature on optimum activity of lipase obtained through solid-state fermentation by Aspergillus niger (strain O-4) and results of lipolytic activity (U/g). Results of mean and standard deviation.

ExperimentpH ()*Temperature (°C) ()Lipolytic activity (U/g)

15 (−1)30 (−1)
26 (0)30 (−1)
37 (+1)30 (−1)
45 (−1)35 (0)
56 (0)35 (0)
67 (+1)35 (0)
75 (−1)40 (+1)
86 (0)40 (+1)
97 (+1)40 (+1)

Conditions of pH obtained using 0.2 M phosphate buffer in the steps of enzyme extraction of the fermented bran and in the enzymatic activity determination.