Isolation and Characterization of Human Lung Lymphatic Endothelial Cells

Figure 2

Fluorescence activated cell sorting. (a)–(c): Representative flow cytometric assay of lymphatic endothelial cells from the human lung (HL-LEC) expanded after double immunomagnetic sorting. (a): negative control represented by the omission of primary antibodies. (b) and (c): virtually 100% of HL-LECs show strong specific positivity for antibodies against Pdn both at passage 3 (P3, (b)) and 8 (P8, (c)). (d): FACS plot of HL-BECs eluted from Pdn immunomagnetic sorting (P4).

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