Simultaneous Treatment with Statins and Aspirin Reduces the Risk of Prostate Cancer Detection and Tumorigenic Properties in Prostate Cancer Cell Lines

Figure 1

Effect of STA, ASA, and the combination of both treatments on prostate cancer cell lines proliferation. Values for proliferation are presented as number of cells (*10⁶) after 7 days of treatment for (a) PC3 and (b) LNCaP cell lines with different conditions: nontreated cells, control (C) and control with DMSO (C(DMSO)), and cells treated with statin (STA), aspirin (ASA), and a combination of both statin and aspirin (STA + ASA) (for further details, see Section 2). The bars represent the mean ± SEM. Values that are significantly different by one-way analysis of variance (ANOVA) from the C(DMSO) group are indicated by , . Western blots of cyclin D1 (37 KDa) and the control α-tubulin (52 KDa) performed on total cell extracts after 7 days of treatment are presented for PC3 (c) and LNCaP (d) cell lines. Densitometry analysis using Image J software is represented below bands as fold change values (versus C(DMSO)).

(a)

(b)

(c)

(d)