Nitric Oxide Protects L-Type Calcium Channel of Cardiomyocyte during Long-Term Isoproterenol Stimulation in Tail-Suspended Rats

Figure 5

Properties of LTCC steady-state activation in cardiomyocytes of 4-week tail-suspended and control rats. (a) Special protocol was designed to analyze LTCC steady-state activation. (b) Steady-state activation curves of LTCC with or without 1 μM ISO treatment in CON and 4 wk SUS groups. is the conductance of LTCC at different testing potential; is the maximum conductance. (c) Steady-state activation curves of LTCC with or without 240 nM NAAN treatments. (d) Steady-state activation curves of LTCC with 240 nM NAAN + 1 μM ISO treatments. (e) Steady-state activation curves of LTCC with 100 μM SNAP. (f) Steady-state activation curves of LTCC with 100 μM SNAP + 1 μM ISO. (g) of steady-state activation curves with different treatments. is the half-activated potential which reflects the open probability of channels. (h) value of steady-state activation curves. is a slope factor which indicates the voltage sensitivity of channels. Data are mean ± SEM; cardiomyocytes from 5 hearts. and versus CON vehicle. and versus CON with ISO treatment.

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