Nitric Oxide Protects L-Type Calcium Channel of Cardiomyocyte during Long-Term Isoproterenol Stimulation in Tail-Suspended Rats

Figure 6

Properties of LTCC steady-state inactivation in cardiomyocytes of 4-week tail-suspended and control rats. (a) Special protocol was designed to analyze LTCC inactivation property. (b) Steady-state inactivation curves of LTCC with or without 1 μM ISO treatment. is at different prepulse potential; is the maximum current. (c) Steady-state inactivation curves of LTCC with or without 240 nM NAAN. (d) Steady-state inactivation curves of LTCC with or without 240 nM NAAN + 1 μM ISO. (e) Steady-state inactivation curves of LTCC with or without 100 μM SNAP. (f) Steady-state inactivation curves of LTCC with or without 100 μM SNAP + 1 μM ISO. (g) of steady-state inactivation curves with different treatments in CON and 4 wk SUS groups. is the prepulse potential at which 50% of channels are available. (h) value of steady-state inactivation curves. is a slope factor which indicates voltage sensitivity of the channels. Data are mean ± SEM; cardiomyocytes from 5 hearts. and versus CON with ISO treatment.

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