[Retracted] N-(1-Pyrenyl) Maleimide Induces Bak Oligomerization and Mitochondrial Dysfunction in Jurkat Cells

Figure 3

NPM induces oligomerization of Bak and Bax. (a) Expression of Bax and Bak in Jurkat and HL60 cells. The cell lysates of Jurkat and HL60 cells were analyzed for the expression of Bax and Bak by Western blot. The effect of NPM on the oligomerization of Bak and Bax in Jurkat (b) and HL60 (c) cells was analyzed by treating the cells with NPM at 0.5–1 μM or with staurosporine (STS) at 500 nM for 3 h. The treated cells were assayed for the presence of Bax and Bak oligomers as described in Section 2. Data shown were from a representative of two independent experiments that gave similar results. Actin served as a loading control. BMH, bismaleimidohexane.

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