[Retracted] N-(1-Pyrenyl) Maleimide Induces Bak Oligomerization and Mitochondrial Dysfunction in Jurkat Cells

Figure 4

Effect of Bak knockdown on NPM-induced apoptosis in Jurkat cells. (a) Expression of Bak in the Jurkat cells that stably express shRNA against Bak (shBak) or against luciferase (shLuc). Expression of Bak in the cell lysates was assayed by Western blot. The effect of Bak knockdown on NPM-induced cytotoxicity (b), loss of mitochondrial membrane potential () (c), the release of cytochrome C from mitochondria to cytosol (d), and caspase activation (e) were analyzed in the Jurkat cells that stably express shRNA against Bak (shBak) or against luciferase (shLuc), as described in the legends to Figures 1 and 2. Data were from a representative of three independent experiments that gave similar results.

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