[Retracted] N-(1-Pyrenyl) Maleimide Induces Bak Oligomerization and Mitochondrial Dysfunction in Jurkat Cells

Figure 6

Effect of caspase-8 inhibition and Bid downregulation on NPM-induced Bak oligomerization. (a) Effect of caspase-8 inhibition on NPM-induced oligomerization of Bak and tBid formation. Jurkat cells were pretreated with 50 μM of caspase-8 inhibitor Z-IETD-FMK for 1 h before being exposed to 0.5 or 1 μM of NPM for 3 h. The cell lysates were then assayed for the Bak oligomers and tBid as described in Section 2. (b) Effect of Bid downregulation on NPM-induced oligomerization of Bak and tBid formation. The Jurkat cells that stably express shRNA against Bid (shBid) or against luciferase (shLuc) were treated with NPM at 0.5–1 μM for 3 h. The cell lysates of the treated cells were assayed for the Bak oligomers and tBid as described in Section 2. Data shown were from a representative of three independent experiments that gave similar results. Actin served as a loading control.

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