Loop Mediated Isothermal Amplification for Detection of Trypanosoma brucei gambiense in Urine and Saliva Samples in Nonhuman Primate Model

Table 1

Detection (%) of parasitological methods, PCR, and LAMP in serum, saliva, and urine determined at weekly time points in vervet monkeys infected with T. b. gambiense.

DPI	Parasito.	PCR		LAMP
		Serum	Saliva	Serum	Saliva	Urine
7	100	100	17	100	33	0
14	83	100	83	100	83	17
21	100	100	100	100	100	33
28	83	100	100	100	100	83
35	67	100	100	100	100	100
42	83	100	83	100	100	100
49	83	100	33	100	100	100
56	83	100	17	100	100	100
63	83	100	17	100	100	83
70	67	100	0	100	100	100
77	83	100	0	100	100	83
84	67	100	0	100	83	83
91	50	83	0	100	83	83
98	67	83	0	100	83	67
105	67	83	0	100	83	67
112	50	83	0	100	83	33
119	50	67	0	100	33	17
126	0	83	0	100	33	17
133	0	67	0	100	17	0
140	0	33	0	83	0	0
147	0	33	0	83	0	0
154	0	17	0	83	0	0
161	0	0	0	83	0	0
168	0	0	0	67	0	0
175	0	0	0	67	0	0
180	0	0	0	33	0	0

Key: Parasito. = parasitological techniques; DPI = days after infection.
There was no amplification in any CSF sample with either LAMP or PCR. There was also no amplification noted in urine using PCR.