Effects of Low-Protein Diets Supplemented with Ketoacid on Expression of TGF-<i>β</i> and Its Receptors in Diabetic Rats

Yang, Xiu; Yang, Ming; Cheng, Ming; Ma, Li-Bin; Xie, Xiang-Cheng; Han, Shuai; Zhang, Bo; Fei, Xiao; Wang, Ming; Mei, Chang-Lin

doi:https://doi.org/10.1155/2015/873519

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Research Article | Open Access

Volume 2015 | Article ID 873519 | https://doi.org/10.1155/2015/873519

Effects of Low-Protein Diets Supplemented with Ketoacid on Expression of TGF-β and Its Receptors in Diabetic Rats

Xiu Yang,¹Ming Yang,²Ming Cheng,²Li-Bin Ma,¹Xiang-Cheng Xie,¹Shuai Han,³Bo Zhang,²Xiao Fei,¹Ming Wang,¹and Chang-Lin Mei²

Academic Editor: Goutam Ghosh Choudhury

Received10 Oct 2015

Accepted26 Nov 2015

Published15 Dec 2015

Abstract

TGF-β₁ has been recognized as a key mediator in DN. This study aimed to observe the effects of low-protein diets supplemented with ketoacid on mRNA and protein expression of TGF-β and TβRI and t TβRII receptors in the renal tissue of diabetic rats. A diabetes model was established in 72 male SD rats. They were then equally randomized to three groups: NPD group, LPD group, and LPD + KA group. Additional 24 male SD rats receiving normal protein diets were used as the control. Eight rats from each group were sacrificed at weeks 4, 8, and 12 after treatment, from which SCr, BUN, serum albumin, and 24 h urinary protein excretion were collected. The expressions of TGF-β₁, TβRI, and TβRII in LPD and LPD + KA groups were significantly lower than those in NPD group and lower in LPD + KA group than those in LPD group. Low-protein diets supplemented with ketoacid have been demonstrated to provide a protective effect on the renal function as represented by reduced SCr, BUN, and urinary protein excretion, probably through downregulating the gene expression of TGF-β₁ and its receptors in LPD + KA group.

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Copyright © 2015 Xiu Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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