Research Article
Evaluation of Genetic Variations in Maize Seedlings Exposed to Electric Field Based on Protein and DNA Markers
Table 1
Electrophoretic banding analysis of proteins of ELF exposed and nonexposed maize seedlings.
| | Rows | Mol. weights | Electric field treatments | | | Lanes |
Band types | | Lane T0 | Lane T1 | Lane T3 | Lane T5 | | KDa | % | KDa | % | KDa | % | KDa | % | |
| | 1 | 186.20 | 1 | 2.27 | 1 | 4.40 | 1 | 9.21 | 1 | 10.54 | M | | 2 | 180.15 | 0 | — | 0 | — | 0 | — | 1 | 11.80 | U | | 3 | 155.00 | 0 | — | 0 | — | 0 | — | 1 | 1.10 | U | | 4 | 151.27 | 0 | — | 1 | 12.00 | 0 | — | 0 | — | U | | 5 | 146.31 | 1 | 10.40 | 0 | — | 1 | 11.20 | 1 | 1.90 | Non-U | | 6 | 131.38 | 0 | — | 0 | — | 0 | — | 1 | 1.66 | U | | 7 | 130.19 | 1 | 1.00 | 1 | 1.09 | 1 | 1.62 | 0 | — | Non-U | | 8 | 118.79 | 0 | — | 0 | — | 0 | — | 1 | 1.11 | U | | 9 | 116.71 | 1 | 22.10 | 1 | 19.60 | 1 | 21.60 | 1 | 24.21 | M | | 10 | 106.42 | 1 | 10.40 | 1 | 14.60 | 1 | 16.90 | 1 | 17.90 | M | | 11 | 96.84 | 0 | — | 0 | — | 1 | 5.14 | 1 | 6.18 | Non-U | | 12 | 89.06 | 1 | 28.30 | 0 | — | 0 | — | 0 | — | U | | 13 | 85.29 | 1 | 32.30 | 1 | 22.50 | 1 | 26.60 | 1 | 28.00 | M | | 14 | 80.16 | 1 | 2.70 | 0 | — | 0 | — | 0 | — | U | | 15 | 78.84 | 0 | — | 1 | 12.66 | 1 | 5.14 | 1 | 2.62 | Non-U | | 16 | 75.55 | 0 | — | 0 | — | 0 | — | 1 | 15.90 | U | | 17 | 69.27 | 1 | 4.66 | 0 | — | 0 | — | 0 | — | U | | 18 | 66.66 | 1 | 13.40 | 0 | — | 0 | — | 0 | — | U | | 19 | 64.77 | 0 | — | 0 | — | 1 | 21.50 | 0 | — | U | | 20 | 63.45 | 0 | — | 1 | 12.60 | 0 | | 0 | — | U | | 21 | 62.91 | 0 | — | 0 | — | 0 | — | 1 | 21.30 | U | | 22 | 62.11 | 1 | 9.39 | 0 | — | 0 | — | 0 | — | Non-U | | 23 | 61.45 | 1 | 22.50 | 1 | 20.10 | 0 | — | 0 | — | Non-U | | 24 | 61.23 | 0 | — | 0 | — | 1 | 21.50 | 0 | — | U | | 25 | 60.91 | 0 | — | 0 | — | 0 | — | 1 | 17.30 | U | | 26 | 36.00 | 0 | — | 0 | — | 0 | — | 1 | 5.97 | U |
| | Number of polypeptide bands of each lane | | 12 | 9 | 11 | 15 | | | Total polypeptide bands | | | | 47 | | | | | | | % of total bands of each lane | | 25.53 | 19.15 | 23.40 | 31.92 | |
| | Types and frequency of polypeptide bands | | Unique (U) | (Non-U) | Polymorphic | Monomorphic (M) | Polymorphism % | | | Number | % | Number | % | Number | % | Number | % |
| | | | 16 | 34.04 | 6 | 12.77 | 46.81 | 22 | 4 | 8.51 | 84.62 |
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