Evaluation of Genetic Variations in Maize Seedlings Exposed to Electric Field Based on Protein and DNA Markers

Table 7

RAPD products of DNA extracted from ELF exposed and nonexposed maize seedlings using 5 random primers.

Primer code	Primers sequence; (5′→3′)	Amplicon lengths (bp)	Electric field treatments						Polymorphic bands						Number of monomorphic bands	%	% of total polymorphism
			Number of scorable bands				Total bands	%	Number of unique bands	%	Number of nonunique bands	%	Number of total polymorphic bands	%
			Lane T0	Lane T1	Lane T2	Lane T3
P-02	GGA CCC AAC C	992–395	3	3	3	4	13	16.67	5	38.46	2	15.39	7	76.92	1	7.69	87.50
P-06	ACC TGA ACG G	929–450	4	3	3	3	13	16.67	7	53.85	1	7.69	9	69.23	1	7.69	90
P-08	GTG TGC CCC A	865–426	3	3	4	4	14	17.95	6	42.86	2	14.29	8	57.14	1	7.14	88.89
P-10	GGT CTA CAC C	764–214	5	4	3	5	17	21.80	15	88.24	1	5.88	16	94.12	—	—	100
P-14	CTT CCC CAA G	800–120	6	3	5	7	21	26.92	18	85.71	1	4.76	19	90.48	—	—	100
Overall total of DNA bands in each treatment			21	16	18	23	78		51	65.35	7	8.97	58	74.36	3	3.85	95.08
%			26.92	20.51	23.08	29.49