Inhibition of mTOR by Rapamycin Results in Auditory Hair Cell Damage and Decreased Spiral Ganglion Neuron Outgrowth and Neurite Formation In Vitro

Figure 2

Effect of rapamycin on auditory HCs by cochlear turns. Photograph of anti-myosin VIIa-labeled OCs. Scale bar 20 μm. (a–f) Untreated OCs (control) and OCs exposed to 10 μM rapamycin demonstrate three rows of OHCs and a single row of IHCs in all cochlear turns. (g–l) OCs treated with increasing concentrations of rapamycin (50 μM and 100 μM, resp.) show a dose dependent loss of OHCs and IHCs.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)